Difference between revisions of "Part:BBa K3332087"
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'''Fig 1. '''pTrc-2 derivative_RBS(B0034)_ECFP(E0020)_terminator(B0015) | '''Fig 1. '''pTrc-2 derivative_RBS(B0034)_ECFP(E0020)_terminator(B0015) | ||
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===Characterization=== | ===Characterization=== | ||
The agarose gel electrophoresis images are shown as below: | The agarose gel electrophoresis images are shown as below: | ||
Revision as of 18:44, 27 October 2020
pTrc-2 derivative-RBS-ECFP-terminator
We use pTrc-2 derivative promoter to express ECFP to check the function of pTrc-2 derivative promoter
With this part, the pTrc-2 derivative promoter can be tested by observing the fluorescence intensity/OD.
Usage and Biology
This part can be used to test that if the pTrc-2 derivative promoter can work. The fluorescence intensity can reflect the strength of the pTrc-2 derivative promoter.
Characterization
The agarose gel electrophoresis images are shown as below:
Fig 2. pTrc-2 derivative_E0420_pSB1C3[BBa_K3332087] digested by Xba I and Pst I (about 1018 bp)
Reference
[1] Chan CT, Lee JW, Cameron DE, Bashor CJ, Collins JJ. 'Deadman' and 'Passcode' microbial kill switches for bacterial containment. Nat Chem Biol. 2016;12(2):82-86. doi:10.1038/nchembio.1979
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 37
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]