Difference between revisions of "Part:BBa K3630025"

Latest revision as of 16:31, 27 October 2020

PCB formation LuxR-OmpC dependent expression system

Figure 1. Light dependent and AHL-LuxR dependent express enzyme and adhesion protein)

The figure shows our design for the Light-sensitive LuxR expression and repression system. We use the constitutive promoter (BBa_J23106) to constitutively express LuxR so that under illuminated conditions or red light conditions our LuxR detection system would still be active. Since we want our bacteria to not produce the digestive enzymes when it’s in the dark: We have incorporated a Lac operon (BBa_K3630002) just downstream of the Constitutive promoter (BBa_J23106) for the LuxR expression system. We then put the Lacl (BBa_K3630020) gene under the OmpC (BBa_K3630015) promoter where OmpR would bind. In a dark environment or far-red illumination, our BREAC would express the Lacl (BBa_K3630020) gene and LacI (BBa_K3630020) would bind to the Lac operon (BBa_K3630002) to repress transcription of our LuxR protein.

Sequence and Features