Difference between revisions of "Part:BBa K3408001"

Revision as of 16:22, 27 October 2020

Phy(ycD)(Phytase)

It is the enzyme which could fix lead efficiently in a middle pH environment.

Because of the limitation of pH, we have to choose the phosphate-solubilizing enzyme which could work efficiently between the pH from pH6.0 to pH7.0. And we have found the Phy(ycD) whose optimum reaction pH is pH6.5. Thus, we added it into the anaerobic regulation circuit, following the P_nar.

Fig.2. Phy (ycD) activity changes with temperature(Wang Xi, et al., Applied and Environmental Biology. 2014)

According to the paper, we could see its optimum reaction temperature is 45℃, Km is 0.213mmol/L and Vmax is 5.55U/mg.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 359
1000
COMPATIBLE WITH RFC[1000]

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 Because of the limitation of pH, we have to choose the phosphate-solubilizing enzyme which could work efficiently <b>between the pH from pH6.0 to pH7.0</b>. And we have found the Phy(ycD) whose <b>optimum reaction pH is pH6.5</b>. Thus, we added it into the anaerobic regulation circuit, following the P<sub>nar</sub>.
-https://2020.igem.org/wiki/images/a/a0/T--NAU-CHINA--Basic-part-2.png
+<html>
+<img src="https://2020.igem.org/wiki/images/a/a0/T--NAU-CHINA--Basic-part-2.png" style="width:40%"/>
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 <span style="text-align:center; display:inline-block;">Fig.2. Phy (ycD) activity changes with temperature(Wang Xi, et al., <i>Applied and Environmental Biology</i>. 2014)</span>