Difference between revisions of "Part:BBa K1362424"
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− | + | We aimed to use this part for circularization of the cyclotide protein. For using this we found that it had 7 nucleotide sequences that made it incompatible for Gibson's assembly/Restriction free cloning. The 7 length sequence makes it out of frame with other components of the composite structure. So without disturbing the restriction site we designed a similar part that overcomes this problem: BBa_K3582021. | |
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Latest revision as of 15:48, 27 October 2020
BsaI restriction site for RFC[105] cloning
This is the reverse complement of a BsaI restriction site headed by an Adenine as a spacer-base to separate the recognition sequence from the outward-lying cutting sequence. It was used by us for scarless golden-gate cloning to fuse inteins to other proteins and thereby implement a variety of possible post-translational modifications.
Information added by Team IISER-Pune-India 2020
Team: IISER-Pune-India 2020
Author: Avadhoot Jadhav
Summary
We aimed to use this part for circularization of the cyclotide protein. For using this we found that it had 7 nucleotide sequences that made it incompatible for Gibson's assembly/Restriction free cloning. The 7 length sequence makes it out of frame with other components of the composite structure. So without disturbing the restriction site we designed a similar part that overcomes this problem: BBa_K3582021.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1