Difference between revisions of "Part:BBa K3468034"

 
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===Usage and Biology===
 
===Usage and Biology===
We use FoldX to judge the thermal stability of T116R and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out what interaction make it become stable.
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[[File:T116R.png|400px|thumb|left|Fig.1 T116R in PyMOL]]
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There is new hydrogen bond increased between T116R and T88, The hydrogen bond is an interaction formed by N, O and H in a protein, which can stabilize the structure of the protein and improve thermal stability.
 
There is new hydrogen bond increased between T116R and T88, The hydrogen bond is an interaction formed by N, O and H in a protein, which can stabilize the structure of the protein and improve thermal stability.
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We use FoldX to judge the thermal stability of T116R and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out what interaction make it become stable.
 +
[[File:T116R.png|400px|thumb|left|Fig.1 T116R in PyMOL]]
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3468034 parameters</partinfo>
 
<partinfo>BBa_K3468034 parameters</partinfo>
 
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Latest revision as of 13:09, 27 October 2020


PETase T116R

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from T to R at 116 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

There is new hydrogen bond increased between T116R and T88, The hydrogen bond is an interaction formed by N, O and H in a protein, which can stabilize the structure of the protein and improve thermal stability.

We use FoldX to judge the thermal stability of T116R and Jmol to find whether there is a new salt bridge. Finally we use Pymol to find out what interaction make it become stable.

Fig.1 T116R in PyMOL