Difference between revisions of "Part:BBa K190015:Experience"
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The part (<partinfo>BBa_K190015</partinfo>) was used in our (<partinfo>BBa_K3380603</partinfo>) construct. '''Figure 1''' shows the effect of As(III) addition 7microM on the transcription of the iSpinach fluorescent RNA aptamer in ''Cupriavidus Metallidurans'' cell-free extract. | The part (<partinfo>BBa_K190015</partinfo>) was used in our (<partinfo>BBa_K3380603</partinfo>) construct. '''Figure 1''' shows the effect of As(III) addition 7microM on the transcription of the iSpinach fluorescent RNA aptamer in ''Cupriavidus Metallidurans'' cell-free extract. | ||
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'''Figure 1.''C. Metallidurans'' cell-free extract containing the construct under the ArsR transcription factor repression expressing iSpinach''' Normalised Fluorescence over time in minutes. 100ng of DNA template was used. The T7 RNA polymerase and 7 μM As(III) were added at time= 0 min. The measurements were done in triplicates. The black line exhibits fluorescence of the construct BBa_K3380500. The green line exhibits fluorescence of the BBa_K3380603 construct with 7 μM As(III) and the blue line exhibits fluorescence with the BBA_K3380603 construct with no metal addition. The details of the construct assembly and the exact concentrations of parts used can be found under the Experience page of the similar construct BBa_K3380500 | '''Figure 1.''C. Metallidurans'' cell-free extract containing the construct under the ArsR transcription factor repression expressing iSpinach''' Normalised Fluorescence over time in minutes. 100ng of DNA template was used. The T7 RNA polymerase and 7 μM As(III) were added at time= 0 min. The measurements were done in triplicates. The black line exhibits fluorescence of the construct BBa_K3380500. The green line exhibits fluorescence of the BBa_K3380603 construct with 7 μM As(III) and the blue line exhibits fluorescence with the BBA_K3380603 construct with no metal addition. The details of the construct assembly and the exact concentrations of parts used can be found under the Experience page of the similar construct BBa_K3380500 | ||
Revision as of 12:40, 27 October 2020
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K190015
BBa_J33201 (Available) E. coli chromosomal ars promoter with arsR repressor gene
BBa_J33203 (Available) E. coli ars promoter, arsR gene and lacZ
BBa_K190023 (Planning) E. coli ars promoter, with RBS site
BBa_K190033 (Planning) E. coli ars promoter, GVP gene cluster
User Reviews
UNIQb755634fc8fd1c8d-partinfo-00000000-QINU
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iGEM Groningen 2009 |
The part (BBa_K190015) was used in our overall arsenic clean up E.coli to activate gas vesicle production upon arsenic detection. The promoter seemed to activate formation of vesicles without addition of arsenic in our tests. Either the ArsR regulator protein is produced in a too low amount to control unwanted expression (in witch case the gene for ArsR has to be upregulated), or the arsenic levels in our water are already sufficient for activation of expression. |
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iGEM Groningen 2009 |
Relative promoter units were calculated from RFP fluorescence upon arsenite induction of the ArsR-promoter. The promoter had an induction of 2.26 (RPU) after induction for 3hrs with 100uM NaAsO2. This is an internal concentration between 0.8-1.2uM As (as seen in Figure 1).
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iGEM Edinburgh 2020 |
The part (BBa_K190015) was used in our (BBa_K3380603) construct. Figure 1 shows the effect of As(III) addition 7microM on the transcription of the iSpinach fluorescent RNA aptamer in Cupriavidus Metallidurans cell-free extract.
Figure 1.C. Metallidurans cell-free extract containing the construct under the ArsR transcription factor repression expressing iSpinach Normalised Fluorescence over time in minutes. 100ng of DNA template was used. The T7 RNA polymerase and 7 μM As(III) were added at time= 0 min. The measurements were done in triplicates. The black line exhibits fluorescence of the construct BBa_K3380500. The green line exhibits fluorescence of the BBa_K3380603 construct with 7 μM As(III) and the blue line exhibits fluorescence with the BBA_K3380603 construct with no metal addition. The details of the construct assembly and the exact concentrations of parts used can be found under the Experience page of the similar construct BBa_K3380500 |
UNIQb755634fc8fd1c8d-partinfo-00000007-QINU