Difference between revisions of "Part:BBa K133050"
 
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Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production.
 
Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production.
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Rgd - His stop is attached at the end of the construct, for protein production.
  
  

Latest revision as of 02:39, 30 October 2008

TetR-CF-RGD-Histop

TetR repressible promoter with ribosomal binding site.

Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production.

Rgd - His stop is attached at the end of the construct, for protein production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1267
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 824
    Illegal BamHI site found at 1335
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1618