Difference between revisions of "Part:BBa K3580101"
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==Characterization==
 
==Characterization==
  
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[[File:T--waseda--monoterpene_2-2-1_parts_and_metabolic_pathways_in_this_experiment_and_a_schematic_diagram_of_the_experiment.png|400px|thumb|center|画像タイトル]]
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[[File:T--waseda--monoterpene_2-2-1_parts_and_metabolic_pathways_in_this_experiment_and_a_schematic_diagram_of_the_experiment.png|800px|thumb|center|Fig. 1 Parts and metabolic pathways in the experiment of monoterpene synthesis and a schematic diagram of the experiment ]]

Revision as of 10:43, 27 October 2020


Ptrc-trGPPS-LS(Limonene synthase)

It is a part composed of trGPPS and limonene synthase whose expression is regulated by Ptrc, and LacI to enable IPTG induction of expression of those enzymes. This part was created and used in a paper reported by Alonso-Gutierrez, Jorge et al. in 2013. Limonene can be synthesized by combining this part with an upstream pathway that can supply the products of the mevalonate pathway (IPP and DMAPP).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1980
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1980
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2366
    Illegal XhoI site found at 4022
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1980
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1980
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Fig. 1 Parts and metabolic pathways in the experiment of monoterpene synthesis and a schematic diagram of the experiment