Difference between revisions of "Part:BBa K3406016"
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| − | The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas protein, and different Cas proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to LbaCas13a and MESR- | + | The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas protein, and different Cas proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to LbaCas13a and MESR-CoV, which could combine with LbaCas13a protein and guide it to target MERS-CoV sequence, thus activating the collateral cleavage of LbaCas13a protein. |
Latest revision as of 16:52, 27 October 2020
LbaCas13a-MERS-CoV-crRNA
The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas protein, and different Cas proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to LbaCas13a and MESR-CoV, which could combine with LbaCas13a protein and guide it to target MERS-CoV sequence, thus activating the collateral cleavage of LbaCas13a protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 49
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 9