Difference between revisions of "Part:BBa K1051301:Design"
 
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===Design Notes===
 
===Design Notes===
−
We get the sequence of the <i>CLN2</i> from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows :
+
We get the sequence of <i>CLN2</i> from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast genome using PCR .The primers are as follows :
  
 
  upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTAGCATCTCATAGCATGGG
 
  upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTAGCATCTCATAGCATGGG

Latest revision as of 14:56, 27 October 2020


clb2 promoter (during G2 phase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 463
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 437
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We get the sequence of CLN2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast genome using PCR .The primers are as follows : 

upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTAGCATCTCATAGCATGGG

downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTACTATAAGATCAATGAAGAGA


Source

CTAGC ATCTC ATAGC ATGGG CATAGAGTTTCAGAAATTTTGCTCTTAATGGAAAATATAA CCTCTTTGGGGAAAAGAGAAATAGATCCTTTCTCAAAAATTTAGAAAGGAAAAAAAATCA AAATATAATAAAATAGATTTTTTTTAGGTTAATTTTTTGCCAATTTAGCTAGACTTTTGA AAAGATCCAAAAATTTACTAAAATTGAATCTTTCTGGTATTAATTTTGTCCTATACCTCA AGAAGTAAAGTATTCTCGAATTGATACTTCTGAAAAGCGAAAGAAATATAGCTAAAGGAT ACTGGTTATTTATTCTTTCTTTTTTTCTTTCTTTTTGATTGAGCCAGTGTATAATAAAAA CCTACTGCGAGCTATTCAATAAATTGACATAGAATATCTGTTGAAGCGGTTCTTTGATTG AGCATCTGTTTTCCTTTTGTATTTTTGTCCTTTTAGATTCTTTTAACGACATTGGAGAAA TTTTAATCATTGTATAAAAACTTCATTCGCTCGTTTGTCAGAAGTTTTTTTATAACTTGC TATTTCCTTCCAAGAAGCCTTTTATTGATTACCCCCTCTCTCTCTTCATTGATCTTATAG

References