Difference between revisions of "Part:BBa K3365000"

Revision as of 09:00, 27 October 2020

dCas9-ω

An RNAP ω subunit, RpoZ is fused to dCas9 C-terminal.

Usage and Biology

The dCas9 is a Cas9 nuclease mutant. Mutations D10A and H840A in the RucC and HNH domains, respectively, abolish cleavage but do not impair DNA binding. The dCas9 provides a simple and robust technology for gene repression and activation, and can target almost any DNA sequence aided by the sgRNA. The ω subunit can activate transcription by recruiting the RNAP holoenzyme. The fusion between dCas9 and ω subunit can activate gene transcription downstream of the protospacer. The CRISPR interference inhibits transcription by sterically blocking the RNA polymerase(RNAP).

Fig:The gene circuit of this part

Fig:The figure above is shown how this part work in bacterial. The geneⅢ mentioned can be changed to any gene sequence depends on designed sgRNA.

Sequence and Features