Difference between revisions of "Part:BBa K3447110"
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Characterization | Characterization | ||
To verify the construction of agrBD, the digestion and agarose gel electrophoresis were performed by a standard protocol (Fig. 1). | To verify the construction of agrBD, the digestion and agarose gel electrophoresis were performed by a standard protocol (Fig. 1). | ||
| − | [[Image: Digestion and electrophoresis of agrBD.jpg|thumb|center| | + | [[Image: Digestion and electrophoresis of agrBD.jpg|thumb|center|300px|<b>Figure 1. Digestion and electrophoresis of agrBD.</b>]]<br> |
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==<b>Design</b>== | ==<b>Design</b>== | ||
===Design Notes=== | ===Design Notes=== | ||
Revision as of 08:42, 27 October 2020
The expression of AIP
This is a part that can produce AIP constitutively.
Usage and Biology
The agrD gene encodes the signaling propeptide, and the agrB gene encodes the peptidase used to process the signaling propeptide. We use these two parts together to produce AIP constitutively.
Characterization
To verify the construction of agrBD, the digestion and agarose gel electrophoresis were performed by a standard protocol (Fig. 1).
Design
Design Notes
We added some synonymous mutations to avoid part rules.
Source
We found this sequence data in GenBank.
References
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 339
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 339 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 339
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 339
- 1000COMPATIBLE WITH RFC[1000]