Difference between revisions of "Part:BBa K3384131:Design"
 
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The promoter of <em>prm1</em> was obtained by PCR amplification using <em>Saccharomyces cerevisiae</em> BY4741 genome as template. The green fluorescent protein(GFP) was obtained by PCR amplification using engineered <em>Saccharomyces cerevisiae</em> BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template.
+
The promoter of <em>prm1</em> was obtained by PCR amplification using <em>Saccharomyces cerevisiae</em> BY4741 genome as template. The green fluorescent protein (GFP) was obtained by PCR amplification using engineered <em>Saccharomyces cerevisiae</em> BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template.
  
  

Latest revision as of 07:31, 27 October 2020

pprm1-GFP-CYC1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1149


Design Notes

The promoter of prm1 was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The green fluorescent protein (GFP) was obtained by PCR amplification using engineered Saccharomyces cerevisiae BY4741-GFP genome as template. The terminator CYC1 was obtained by PCR amplification using pCas9 plasmid as template.


Source

Saccharomyces cerevisiae