Difference between revisions of "Part:BBa K3645016:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The scaffold of sgRNA already contains a terminator domain, so to use this part we only need to replace the guide sequence | + | The scaffold of sgRNA already contains a terminator domain, so to use this part we only need to replace the guide sequence. |
Latest revision as of 07:11, 27 October 2020
sgRNA scaffold
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 135
Illegal BsaI.rc site found at 37
Design Notes
The scaffold of sgRNA already contains a terminator domain, so to use this part we only need to replace the guide sequence.
Source
From Peking iGEM 2019