Difference between revisions of "Part:BBa K3447000:Design"

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===Source===
 
===Source===
  
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We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.This gene is derived from <i>Escherichia coli</i> (strain: A2363), we get the genomic sequence by NCBI.<br>
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We get the genomic sequence by NCBI and ordered the part DNA from a synthesis company.
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https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075<br>
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https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164<br>
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We ordered the part DNA from a synthesis company.
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===References===
 
===References===

Revision as of 06:57, 27 October 2020


luxI, an autoinducer synthetase for AHL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a repetitive sequence between cvaA and cvaB gene. In order not to increase restriction sites, we modified the natural gene and added some nonsense mutations, and other than that there is no need to pay attention.

Source

We get the genomic sequence by NCBI and ordered the part DNA from a synthesis company.

References