Difference between revisions of "Part:BBa K3406003"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3406003 short</partinfo>
 
<partinfo>BBa_K3406003 short</partinfo>
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<p>LbaCas13a is a member of Cas13 protein family.It was originally found in Prevotella sp.
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MA2016 and has the common characteristic of Type VI RNA-targeting CRISPR–Cas system’s proteins, which means it could be activated by target RNA and could cleave bystander single strained RNAs. This feature could be used to target RNA and thus to detect RNA and to edit RNA.
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The coding sequence of LbaCas13a protein.It contains rare codons, so it needs to be expressed in <i>E. coli</i> strains which is rich in corresponding tRNAs like <i>E. coli</i> Rosetta2(DE3)pLysS.
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Besides,different Cas13 protein has different base cutting preference,and the preference of CcaCas13b is Poly A/AC
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</p>
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<partinfo>BBa_K3406003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3406003 SequenceAndFeatures</partinfo>

Latest revision as of 06:23, 27 October 2020

LbaCas13a coding sequence

The coding sequence of LbaCas13a protein.It contains rare codons, so it needs to be expressed in E. coli strains which is rich in corresponding tRNAs like E. coli Rosetta2(DE3)pLysS.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2168
    Illegal BglII site found at 3683
    Illegal BamHI site found at 95
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1738
    Illegal AgeI site found at 2815
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 409
    Illegal BsaI.rc site found at 1492