Difference between revisions of "Part:BBa K3630025"
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constitutively promote PCB , which requires ho1 and pcyA, ho1 change heme to biliverdin and pcyA change biliverdin to PCB, constitutively promote Cph8 receptor which is necessary to change the EnvZ and transfer phosphoryl to OmpR when it is exposed in a dark environment. So, the OmpR can bind to the OmpC promoter.
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      <img src="https://2020.igem.org/wiki/images/1/12/T--UM_Macau--_Light_dependent_and_AHL-LuxR_dependent_express_enzyme_and_adhesion_protein.jpg" class= "center" width="800"
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<p style="text-align: justify; font-size: 14px; font-family: MuliLight; color: black; margin-left: auto; margin-right: auto;"><b>Figure 1.</b> Light dependent and AHL-LuxR dependent express enzyme and adhesion protein)</p>
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The figure shows our design for the Light-sensitive LuxR expression and repression system. We use the constitutive promoter (BBa_J23106) to constitutively express LuxR so that under illuminated conditions or red light conditions our LuxR detection system would still be active. Since we want our bacteria to not produce the digestive enzymes when it’s in the dark: We have incorporated a Lac operon (BBa_K3630002) just downstream of the Constitutive promoter (BBa_J23106) for the LuxR expression system. We then put the Lacl (BBa_K3630020) gene under the OmpC (BBa_K3630015) promoter where OmpR would bind. In a dark environment or far-red illumination, our BREAC would express the Lacl (BBa_K3630020) gene and LacI (BBa_K3630020) would bind to the Lac operon (BBa_K3630002) to repress transcription of our LuxR protein.
  
 
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Revision as of 16:30, 27 October 2020


PCB formation LuxR-OmpC dependent expression system

HTML img Tag

Figure 1. Light dependent and AHL-LuxR dependent express enzyme and adhesion protein)

The figure shows our design for the Light-sensitive LuxR expression and repression system. We use the constitutive promoter (BBa_J23106) to constitutively express LuxR so that under illuminated conditions or red light conditions our LuxR detection system would still be active. Since we want our bacteria to not produce the digestive enzymes when it’s in the dark: We have incorporated a Lac operon (BBa_K3630002) just downstream of the Constitutive promoter (BBa_J23106) for the LuxR expression system. We then put the Lacl (BBa_K3630020) gene under the OmpC (BBa_K3630015) promoter where OmpR would bind. In a dark environment or far-red illumination, our BREAC would express the Lacl (BBa_K3630020) gene and LacI (BBa_K3630020) would bind to the Lac operon (BBa_K3630002) to repress transcription of our LuxR protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1783
    Illegal NheI site found at 1806
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4088
    Illegal XhoI site found at 2195
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 296