Difference between revisions of "Part:BBa K118011:Experience"

(→‎Applications of BBa_K118011)
(→‎User Reviews)
Line 9: Line 9:
 
===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K118011 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K118011 StartReviews</partinfo>
−
<!-- Template for a user review
+
 
 
{|width='80%' style='border:1px solid gray'
 
{|width='80%' style='border:1px solid gray'
 
|-
 
|-
 
|width='10%'|
 
|width='10%'|
−
<partinfo>BBa_K118011 AddReview number</partinfo>
+
<partinfo>BBa_K118011 1</partinfo>
−
<I>Username</I>
+
<I>Kun</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
Enter the review inofrmation here.
+
<br>
 +
[[Image:II09_CRP-GFP fluor different media.jpg|500px|right]]
 +
Cells with BBa_K200018 were grown overnight in various different media, and the GFP fluorescence was measured. <br>
 +
<br>
 +
After overnight culture, the corrected fluorescence of glucose is almost negligible, showing that glucose represses the PcstA promoter strongly.  <br>
 +
<br>
 +
For all the other secondary carbon sources, 10% Casamino Acids in M9 shows the highest corrected fluorescence at 22000.<br>
 +
<br>
 +
For more information, go to the Imperial iGEM 2009 <i>E.ncapsulator</i> project page on
 +
[http://2009.igem.org/Team:Imperial_College_London/Wetlab/Results/CRP_and_Media BBa_K200018 testing results] <br>
 +
<br>
 
|};
 
|};
−
<!-- End of the user review template -->
+
 
 
<!-- DON'T DELETE --><partinfo>BBa_K118011 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K118011 EndReviews</partinfo>

Revision as of 00:40, 22 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K118011

Preliminary tests of this part were conducted using the reporter gene xylE (BBa_K118021). Strong repression occurred in the presence of glucose, and partial repression in the presence of high concentrations of higher sugars. Results can be found [http://2008.igem.org/Team:Edinbrugh/Results/PcstA-xylE here].

User Reviews

UNIQdc01c6d9982a97e5-partinfo-00000000-QINU

BBa_K118011 1 Not understood Kun


II09 CRP-GFP fluor different media.jpg

Cells with BBa_K200018 were grown overnight in various different media, and the GFP fluorescence was measured.

After overnight culture, the corrected fluorescence of glucose is almost negligible, showing that glucose represses the PcstA promoter strongly.

For all the other secondary carbon sources, 10% Casamino Acids in M9 shows the highest corrected fluorescence at 22000.

For more information, go to the Imperial iGEM 2009 E.ncapsulator project page on [http://2009.igem.org/Team:Imperial_College_London/Wetlab/Results/CRP_and_Media BBa_K200018 testing results]

;

UNIQdc01c6d9982a97e5-partinfo-00000002-QINU