Difference between revisions of "Part:BBa K3425070"

Latest revision as of 23:33, 26 October 2020

Modular outer membrane display construct for nanobody specific to HSP biomarker

This composite part serves for the display of nanobodies on the outer surface of E. coli. Display of nanobodies on the surface is useful for testing their functionality.

BBa_K3425070 is designed as a modular system of six components. All components are tailored for iGEM Type IIS assembly standard. In contrast to standard iGEM Type IIS assembly, the coding sequence is split into three parts. The middle nanobody module can be easily exchanged for nanobody of different specificity. In this case the nanobody, B-F10 (BBa_K3425043), is specific to the Mycobacterium tuberculosis biomarker HSP16.3 (BBa_K3425041).

Part number	Name	Description
BBa_J23108	promoter	Constitutive promoter of medium strength.
BBa_K3425032	RBS	RBS tailored for Type IIS assembly.
BBa_K3425042	pelB	Signal peptide directing the fusion protein to the periplasm.
BBa_K3425043	B-F10	Exchangable Module. Nanobody specific for Mycobacterium tuberculosis biomarker HSP 16.3.
BBa_K3425044	ehaA	EhaA can be used as an outer membrane display system for E. coli
BBa_B0015	terminator	Double terminator. E. coli

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1036
Illegal AgeI site found at 625
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3425070 short</partinfo>
-This composite part serves for display of nanobodies on the outer surface of <i>E. coli</i>. Display of nanobodies on the surface is useful for testing of their functionality. BBa_K3425070 is designed as a modular system of six components. All components are tailored for <html><a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">iGEM Type IIS assembly standard</a></html>. In contrast to standard iGEM Type IIS assembly, coding sequence is split into three parts. The middle nanobody module can be easily exchanged for nanobody of different specificity. In this case the nonabody [[Part:BBa_K3425043|<partinfo>BBa_K3425043</partinfo>]] is specific to the <i>Mycobacterium tuberculosis</i> biomarker [[Part:BBa_K3425041|<partinfo>BBa_K3425041</partinfo>]].
+This composite part serves for the display of nanobodies on the outer surface of <i>E. coli</i>. Display of nanobodies on the surface is useful for testing their functionality.
+BBa_K3425070 is designed as a modular system of six components. All components are tailored for <html><a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">iGEM Type IIS assembly standard</a></html>. In contrast to standard iGEM Type IIS assembly, the coding sequence is split into three parts. The middle nanobody module can be easily exchanged for nanobody of different specificity. In this case the nanobody, B-F10 (<partinfo>BBa_K3425043</partinfo>), is specific to the <i>Mycobacterium tuberculosis</i> biomarker HSP16.3 (<partinfo>BBa_K3425041</partinfo>).
 {{CSS/DNA_Table}}