Difference between revisions of "Part:BBa K3331001"
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<partinfo>BBa_K3331001 short</partinfo>
 
<partinfo>BBa_K3331001 short</partinfo>
  
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UTP-glucose-1-phosphate uridylyltransferase (GalU) carries out a key step in the generation of UDP-D-glucuronate.This part catalyzes the addition of UTP to &#945;-D-glucose 1-phosphate to yield UDP-D-glucose.This sequence comes from the genome of E.coli and we optimized the codon of this DNA sequence so that it could be expressed more efficiently in Bacillus subtilis.
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galU [Escherichia coli str. K-12 substr. MG1655]
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Encodes Glucose-1-phosphate uridylyltransferase, or UGPase, which catalyzes the production of UDP-glucose from glucose-1-phosphate and UTP. .This sequence comes from the genome of E.coli and we optimized the codon of this DNA sequence so that it could be expressed more efficiently in Bacillus subtilis.
  
  

Revision as of 10:45, 27 October 2020


E.coli galU

galU [Escherichia coli str. K-12 substr. MG1655] Encodes Glucose-1-phosphate uridylyltransferase, or UGPase, which catalyzes the production of UDP-glucose from glucose-1-phosphate and UTP. .This sequence comes from the genome of E.coli and we optimized the codon of this DNA sequence so that it could be expressed more efficiently in Bacillus subtilis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

We cloned and confirmed the target fragment by PCR. The length of our fragment is 981bp. The following results showed the successful result.

Figure 1:PCR confirmation of the target fragment.