Difference between revisions of "Part:BBa K3362004:Design"

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===References===
 
===References===
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http://labs.biology.ucsd.edu/zhao/CRISPR_web/5_prime_ribozyme_design.html  
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http://labs.biology.ucsd.edu/zhao/CRISPR_web/5_prime_ribozyme_design.html;
 
Gao Y, Zhao Y (2014). Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. Journal of Integrative Plant Biology, 56(4), 343–349. doi:10.1111/jipb.12152
 
Gao Y, Zhao Y (2014). Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. Journal of Integrative Plant Biology, 56(4), 343–349. doi:10.1111/jipb.12152

Revision as of 20:38, 26 October 2020


Hammerhead Ribozyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The first bases are variable, because they are dependent on the bases that are downstream of the ribozyme.


Source

Artificically synthesized

References

http://labs.biology.ucsd.edu/zhao/CRISPR_web/5_prime_ribozyme_design.html; Gao Y, Zhao Y (2014). Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. Journal of Integrative Plant Biology, 56(4), 343–349. doi:10.1111/jipb.12152