Difference between revisions of "Part:BBa K2924026"
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The DNA sequence of the gene was acquired by reverse-translating the amino acid sequence. Further, in order to reach optimal heterologous expression in <i>Escherichia coli</i> the DNA sequence was designed with optimized codons and synthesized commercially. | The DNA sequence of the gene was acquired by reverse-translating the amino acid sequence. Further, in order to reach optimal heterologous expression in <i>Escherichia coli</i> the DNA sequence was designed with optimized codons and synthesized commercially. | ||
| − | <img src="https://2020.igem.org/wiki/images/9/9c/T--Puiching_Macau--bedsheet_chart.png" | + | <h2>Fire Retardancy Test</h2> |
| + | <p> The test subject(Figure 1.) was in the size of bed sheet in 300*130mm and the average Moisture content% is 13.55%-14.37%. As demonstrated on Fig. 1, all of our engineered flame retardant displayed a significant improvement compared with the water control.</p> | ||
| + | <img width="500" src="https://2020.igem.org/wiki/images/9/9c/T--Puiching_Macau--bedsheet_chart.png"> | ||
| + | <p> Figure 1. Vertical Burning Test using bedsheet</p> | ||
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Revision as of 20:32, 26 October 2020
α-s1-casein
The milk protein α-s1-casein from Bos taurus
Usage and Biology
α-s1-casein (CSN1S1), also named B5B3R8_BOVIN1is a gene coding for a protein product and has the GeneID: 282208, which originates from
Caseins are among the most abundant proteins in native cow's milk. They are phosphoproteins and make up approximately 80% of total dairy protein mass. The milk proteins and their proportions are important to the parameters of milk manufacturing and the quality of milk protein.
With a length of 17508 bp and 19 exons the bovine α-s1-casein gene is the most abundant one of the bovine caseins (12-15 g/L)3. The α-s1-casein protein may contain up to eight serine monophosphate residues, which cluster in a hydrophilic domain between amino acids 43–80 and, through modelling studies, are thought to be connected to a hydrophobic domain (amino acids 100–199) by helical and sheet secondary structures4. In the ability of milk to carry calcium phosphate, α-s1-casein also performs an significant part5.
The DNA sequence of the gene was acquired by reverse-translating the amino acid sequence. Further, in order to reach optimal heterologous expression in Escherichia coli the DNA sequence was designed with optimized codons and synthesized commercially.
The test subject(Figure 1.) was in the size of bed sheet in 300*130mm and the average Moisture content% is 13.55%-14.37%. As demonstrated on Fig. 1, all of our engineered flame retardant displayed a significant improvement compared with the water control. <img width="500" src=" Figure 1. Vertical Burning Test using bedsheet </html>
Sequence and Features
[1]: https://www.uniprot.org/uniprot/B5B3R8
[2]: https://www.ncbi.nlm.nih.gov/gene/?term=282208
[3]: Boland, Mike, Harjinder Singh, and Abby Thompson, eds. Milk proteins: from expression to food. Academic Press, 2014.
[4]: Sheehy, P. A., et al. "Significance, origin and function of bovine milk proteins: the biological implications of manipulation or modification." Milk Proteins. Academic Press, 2008. 81-106.
[5]: Chanat, E., J. Cell Sci., 112, 3399-3412 (1999)
Fire Retardancy Test
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References