Difference between revisions of "Part:BBa K3331009"

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In order to increase the production of exopolysaccharides (EPSs), we altered the levels of enzymes in the central metabolism. We selected and overexpressed two key enzymes, galU and pgmA at the same time. And we found we successfully enhance the production of EPSs in E.coli.DH5α
 
In order to increase the production of exopolysaccharides (EPSs), we altered the levels of enzymes in the central metabolism. We selected and overexpressed two key enzymes, galU and pgmA at the same time. And we found we successfully enhance the production of EPSs in E.coli.DH5α
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<div>[[File:T--XJTU-China--BBMap |400px|thumb|center|<b>Figure 1:</b>Plasmid we have constructed for galU&pgmA validations. ]]</div>
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<div>[[File:T--XJTU-China--BBMap.png |400px|thumb|center|<b>Figure 1:</b>Plasmid we have constructed for galU&pgmA validations. ]]</div>

Revision as of 18:53, 26 October 2020


B.S galU+B.S pgmA

The yield of EPS can be significantly increased by overexpressing two key enzymes galU and pgmA at the same time. Reference：Levander Fredrik et al. Applied and environmental microbiology,2002,68(2).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Overview

In order to increase the production of exopolysaccharides (EPSs), we altered the levels of enzymes in the central metabolism. We selected and overexpressed two key enzymes, galU and pgmA at the same time. And we found we successfully enhance the production of EPSs in E.coli.DH5α

Figure 1:Plasmid we have constructed for galU&pgmA validations.