Difference between revisions of "Part:BBa K3396016"
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− | + | Dual luciferase reporter plasmid to indicate the target protein abundance. The coding sequence of target protein can be inserted into the replaceable region with Golden Gate assembly, the ratio Fluc/Rluc shall indicate the normalized protein amount of the target. | |
+ | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
− | <partinfo> | + | <partinfo>BBa_K3396016 SequenceAndFeatures</partinfo> |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K3396016 parameters</partinfo> |
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Revision as of 23:38, 26 October 2020
CMV-replaceable-Fluc-P2A-Rluc
Dual luciferase reporter plasmid to indicate the target protein abundance. The coding sequence of target protein can be inserted into the replaceable region with Golden Gate assembly, the ratio Fluc/Rluc shall indicate the normalized protein amount of the target.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 871
Illegal NgoMIV site found at 2215
Illegal NgoMIV site found at 2236
Illegal AgeI site found at 1939 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3102
Illegal SapI.rc site found at 2121