Difference between revisions of "Part:BBa K3505017"
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===Verification of Cloning=== | ===Verification of Cloning=== | ||
− | [[File:T--Thessaly-terminator-digestion.png|700px|thumb|none|<i><b>Fig.3:</b>(U=Uncut C=Cut) Restriction digestion of Terminator (C3 )with : | + | [[File:T--Thessaly--terminator-teliko-digestion.png|700px|thumb|none|<i><b>Fig.3:</b>(U=Uncut C=Cut) Restriction digestion of Terminator (C3 )with : |
EcoRI & PstI ( C3 ), Expected bands : 2029 + 209 bp , Positive result : C3</i>]] | EcoRI & PstI ( C3 ), Expected bands : 2029 + 209 bp , Positive result : C3</i>]] |
Revision as of 21:35, 26 October 2020
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Double Terminator GB compatible with B6-C1
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Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B6-C1.