Difference between revisions of "Part:BBa K3482018"

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(→‎Usage and Biology)
 
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Colicin E2 is an endonuclease that cuts on both single and double-stranded DNA. It has no specific cutting site. Colicins are toxins that can be produced by E.coli and closely related bacteria.
 
Colicin E2 is an endonuclease that cuts on both single and double-stranded DNA. It has no specific cutting site. Colicins are toxins that can be produced by E.coli and closely related bacteria.
  
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We will use this part in a Kill-switch with the IM2 protein, which provides immunity to Colicin E2.
+
We will use this part in a Kill switch with the IM2 protein, which provides immunity to Colicin E2.
  
 
===Characterization===
 
===Characterization===

Latest revision as of 02:15, 28 October 2020


IPTG and heat-repressible miniColicin toxin

MiniColicin E2 version regulated by a heat-repressible termosensor and activited by IPTG under a pTac promotor

Usage and Biology

Colicin E2 is an endonuclease that cuts on both single and double-stranded DNA. It has no specific cutting site. Colicins are toxins that can be produced by E.coli and closely related bacteria.

We will use this part in a Kill switch with the IM2 protein, which provides immunity to Colicin E2.

Characterization

All the data concerning the characterization of that part can be found in the composite part : BBa_K3482017 .


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 539
    Illegal PstI site found at 579
    Illegal AgeI site found at 155
  • 1000
    COMPATIBLE WITH RFC[1000]