Difference between revisions of "Part:BBa K115003:Experience"

Revision as of 21:21, 27 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K115003

The parts were tested in luciferase constructs, by measuring luciferase activity after growing at certain temperatures. The fold increase of luciferase activity between cultures of cells grown at 20ºC and 37ºC is displayed in figure 1.

In this figure, 12 (the leftmost) is construct K115012, the reference strain, which has no thermosensitive RNA, but just B0032 as ribosome binding site. This is to compare for normal temperature induced behaviour in cells. The other constructs from 29-36 are K115029 to K115036, please look at the experience tab of these constructs to see which thermometer RNA they contain.

For more documentation, visit our wiki [http://2008.igem.org/Team:TUDelft (link)].

User Reviews

UNIQd0e1b859cd5aaf56-partinfo-00000000-QINU UNIQd0e1b859cd5aaf56-partinfo-00000001-QINU

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 <I>Username</I>
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-Enter the review inofrmation here.
+Team BYU_Provo 2011 performed random mutagenesis on the <i>Listeria</i> thermosensor and produced six versions of the thermosensor with tighter temperature ranges. See the following parts for details:
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619890 BBa_K619890]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619891 BBa_K619891]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619892 BBa_K619892]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619893 BBa_K619893]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619894 BBa_K619894]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K619895 BBa_K619895]
 |};
 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K115003 EndReviews</partinfo>