Difference between revisions of "Part:BBa K3505023"
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<partinfo>BBa_K3505023 short</partinfo> | <partinfo>BBa_K3505023 short</partinfo> | ||
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===Verification of Cloning=== | ===Verification of Cloning=== | ||
[[File:T--Thessaly--FFAR2-ECFP-digestion.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction Digestion of FFAR2:V2tail:TCS with BamHI, Expected bands : 3204 bp, Positive Result:C2,C3</i>]] | [[File:T--Thessaly--FFAR2-ECFP-digestion.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction Digestion of FFAR2:V2tail:TCS with BamHI, Expected bands : 3204 bp, Positive Result:C2,C3</i>]] | ||
+ | |||
+ | ===Source=== | ||
+ | Synthesized by IDT. | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
<partinfo>BBa_K3505023 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3505023 SequenceAndFeatures</partinfo> |
Revision as of 19:12, 27 October 2020
FFAR2:V2tail:TCS GB compatible with B2-B3
Usage and Biology
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B2-B5.
Verification of Cloning
Source
Synthesized by IDT.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 284
Illegal PstI site found at 864 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 284
Illegal PstI site found at 864 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 106
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 284
Illegal PstI site found at 864 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 284
Illegal PstI site found at 864
Illegal NgoMIV site found at 364 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 560
Illegal SapI site found at 815
Illegal SapI.rc site found at 17