Difference between revisions of "Part:BBa K3505023"
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[[File:T--Thessaly--FFAR2-ECFP-digestion.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction Digestion of FFAR2:V2tail:TCS with BamHI, Expected bands : 3204 bp, Positive Result:C2,C3</i>]]
 
[[File:T--Thessaly--FFAR2-ECFP-digestion.png|700px|thumb|none|<i><b>Fig.1:</b>(U=Uncut C=Cut) Restriction Digestion of FFAR2:V2tail:TCS with BamHI, Expected bands : 3204 bp, Positive Result:C2,C3</i>]]
  
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Revision as of 15:00, 26 October 2020


FFAR2:V2tail:TCS GB compatible with B2-B3

long


Usage and Biology

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B2-B5.

Figure 1.The overhangs of this part in the Golden Braid Grammar.
Figure 2. The level 0 module : pupd2-FFAR2:V2tail:TCS (illustration from SnapGene)

Verification of Cloning

Fig.1:(U=Uncut C=Cut) Restriction Digestion of FFAR2:V2tail:TCS with BamHI, Expected bands : 3204 bp, Positive Result:C2,C3

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 284
    Illegal PstI site found at 864
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 284
    Illegal PstI site found at 864
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 106
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 284
    Illegal PstI site found at 864
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 284
    Illegal PstI site found at 864
    Illegal NgoMIV site found at 364
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 560
    Illegal SapI site found at 815
    Illegal SapI.rc site found at 17