Difference between revisions of "Part:BBa K3527000"
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The phoA promoter is repressed by high phosphate concentrations. Under phosphate limited conditions, phoA promoter (basic parts BBa_K3527000) is activated. There are the other two phoA promoter sequence (basic parts: (BBa_K1139200 and BBa_K737023) which are much shorter than that of BBa_K3527000, as indicated by the following figure 1. | The phoA promoter is repressed by high phosphate concentrations. Under phosphate limited conditions, phoA promoter (basic parts BBa_K3527000) is activated. There are the other two phoA promoter sequence (basic parts: (BBa_K1139200 and BBa_K737023) which are much shorter than that of BBa_K3527000, as indicated by the following figure 1. | ||
[[File:T--Shanghai_HS-BBa_K3527000 fig 0.jpg|500px|thumb|center|Figure 1]] | [[File:T--Shanghai_HS-BBa_K3527000 fig 0.jpg|500px|thumb|center|Figure 1]] | ||
| + | |||
| + | ===Engineering Success=== | ||
| + | Regulation of phoA promoter by phosphate concentration | ||
| + | The function of phoA promoter was evaluated by the regulation of transcription of the gene of enhanced green fluorescent protein (EGFP). Using this reporting system, we showed that compared with the control, the bacteria containing PhoA-EGFP showed a significant fluorescence intensity difference (Figure 1) in the absence of phosphate in the medium. When the phosphate concentration added into the culture, the fluorescence intensity decreased to a level similar to the control culture. | ||
| + | |||
| + | [[File:T--Shanghai_HS-BBa_K3527000 fig 1.jpg|500px|thumb|center|Figure 2]] | ||
Revision as of 13:39, 26 October 2020
PhoA Promoter
PhoA Promoter
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Contribution
The phoA promoter is repressed by high phosphate concentrations. Under phosphate limited conditions, phoA promoter (basic parts BBa_K3527000) is activated. There are the other two phoA promoter sequence (basic parts: (BBa_K1139200 and BBa_K737023) which are much shorter than that of BBa_K3527000, as indicated by the following figure 1.
Engineering Success
Regulation of phoA promoter by phosphate concentration The function of phoA promoter was evaluated by the regulation of transcription of the gene of enhanced green fluorescent protein (EGFP). Using this reporting system, we showed that compared with the control, the bacteria containing PhoA-EGFP showed a significant fluorescence intensity difference (Figure 1) in the absence of phosphate in the medium. When the phosphate concentration added into the culture, the fluorescence intensity decreased to a level similar to the control culture.
