Difference between revisions of "Part:BBa K3606044"
 
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===Usage and Biology===
 
===Usage and Biology===
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<p>luxpR_CaAP is a composite part constructed to correlate the quorum-sensing module and the calcium absorption module of our project.The promoter luxpR is activated by the heterodimer of luxI and luxR. When the population of our bacteria is low,the amount of the heterodimer is not enough, thus the luxpR promoter cannot initiate expression of downstream genes.</p>
+
<p>luxpR_CaAP is a composite part constructed to correlate the quorum-sensing module and the calcium absorption module of our project.The promoter luxpR is activated by a complex of which the formation requires both enough expression of luxI and luxR. When the population of our bacteria is low,the amount of the complex is not enough, thus the luxpR promoter cannot initiate expression of downstream genes.</p>
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<p>When the population of bacteria reaches a certain level, the heterodimer triggers the luxpR promoter, so that our engineered probiotics will express and secrete CaAP and play a role in facilitating calcium absorption.</p>
+
<p>When the population of bacteria reaches a certain level, the AHL-luxR triggers the luxpR promoter, so that our engineered probiotics will express and secrete CaAP and play a role in facilitating calcium absorption.</p>
 
<p>See more information about luxI-luxR-luxpR regulating system on BBa_K3606100.</p>
 
<p>See more information about luxI-luxR-luxpR regulating system on BBa_K3606100.</p>
  

Latest revision as of 14:16, 26 October 2020


luxPR driven CaAP

CaAP(Calcium Absorption Peptide) regulated by the quorum-sensing promoter luxpR(HS100).

Usage and Biology

luxpR_CaAP is a composite part constructed to correlate the quorum-sensing module and the calcium absorption module of our project.The promoter luxpR is activated by a complex of which the formation requires both enough expression of luxI and luxR. When the population of our bacteria is low,the amount of the complex is not enough, thus the luxpR promoter cannot initiate expression of downstream genes.

When the population of bacteria reaches a certain level, the AHL-luxR triggers the luxpR promoter, so that our engineered probiotics will express and secrete CaAP and play a role in facilitating calcium absorption.

See more information about luxI-luxR-luxpR regulating system on BBa_K3606100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 142
  • 1000
    COMPATIBLE WITH RFC[1000]