Difference between revisions of "Part:BBa K190021"

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SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.  
 
SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.  
<center>https://2020.igem.org/File:T--CSU_CHINA--Contribution_SmtA.png</center><br>
 
  
 
===Usage and Biology===
 
===Usage and Biology===
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===Supplementary Experiment===
 
===Supplementary Experiment===
Group: iGEM20_CSU_CHINA (2020-10-26)
+
Group: iGEM20_CSU_CHINA (2020-10-26)<br>
Author: Hao Zhou
+
Author: Hao Zhou<br>
 
Summary:  
 
Summary:  
 +
<center>https://2020.igem.org/File:T--CSU_CHINA--Contribution_SmtA.png</center><br>
  
  

Revision as of 13:03, 26 October 2020

SmtA

SmtA is a metallothionein that binds Zn (II) ions, it is able to bind 3-4 ions per protein.

Usage and Biology

The gene was functionally expressed in E. coli. The protein can be used to accumulate Zn(II) intracellular.

Supplementary Experiment

Group: iGEM20_CSU_CHINA (2020-10-26)
Author: Hao Zhou
Summary:

https://2020.igem.org/File:T--CSU_CHINA--Contribution_SmtA.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 111
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 111
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 111
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 111
    Illegal AgeI site found at 139
  • 1000
    COMPATIBLE WITH RFC[1000]