Difference between revisions of "Part:BBa K3396004"
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Usage and Biology | Usage and Biology | ||
FKBP is a kind of protein which can bind with FRB induced by rapamycin. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part could target GFP and bind TRIM21-FRB thought the FRB-FKBP interaction and lead to the degradation induced by ubiquitin-proteasome. Here is the mechanism of the recombined TRIM21-FRB: | FKBP is a kind of protein which can bind with FRB induced by rapamycin. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part could target GFP and bind TRIM21-FRB thought the FRB-FKBP interaction and lead to the degradation induced by ubiquitin-proteasome. Here is the mechanism of the recombined TRIM21-FRB: | ||
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1. The GFPnano tagged with FRB combines with targeted protein. | 1. The GFPnano tagged with FRB combines with targeted protein. | ||
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2. TRIM21-DocS connect Coh2-GFPnano-target through the rapamycin induced DocS-Coh2 interaction. | 2. TRIM21-DocS connect Coh2-GFPnano-target through the rapamycin induced DocS-Coh2 interaction. | ||
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3. The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21. | 3. The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21. | ||
Revision as of 12:34, 26 October 2020
GFPnano-FKBP-FKBP
According to the mechanism of the degradation conduced by TRIM21, we designed this Part to make a contrast with FKBP-FKBP-GFPnano.
Usage and Biology
Usage and Biology FKBP is a kind of protein which can bind with FRB induced by rapamycin. GFPnano is the nanobody of GFP, a green fluorescent protein, which can be observed easily. This part could target GFP and bind TRIM21-FRB thought the FRB-FKBP interaction and lead to the degradation induced by ubiquitin-proteasome. Here is the mechanism of the recombined TRIM21-FRB:
1. The GFPnano tagged with FRB combines with targeted protein.
2. TRIM21-DocS connect Coh2-GFPnano-target through the rapamycin induced DocS-Coh2 interaction.
3. The targeted protein is degraded by ubiquitin-proteasome system recruited by TRIM21.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 35