Difference between revisions of "Part:BBa K3396007"
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<partinfo>BBa_K3396007 short</partinfo> | <partinfo>BBa_K3396007 short</partinfo> | ||
| − | This part is the truncated version of human Trim21 coding sequence. We | + | This part is the truncated version of human Trim21 coding sequence. We removed the PRYSPRY domain containing 268-475 aa of the original Trim21 to allow further engineering. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 18:14, 26 October 2020
Truncated Trim21
This part is the truncated version of human Trim21 coding sequence. We removed the PRYSPRY domain containing 268-475 aa of the original Trim21 to allow further engineering.
Usage and Biology
BBa_K3396007 is the core of the newly registered Predator Pro system. Trim 21 comes from the family of TRIM. We truncated the Trim21 protein to maintain its RING domain, B box domain and coiled coli domain, while the antibody binding PRYSPRY domain was removed. This truncate protein maintained the E3 ubiquitin ligase activity, and provided an open interface for other protein dimerization pairs to be added. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 204
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 161
- 1000COMPATIBLE WITH RFC[1000]