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Revision as of 17:30, 26 October 2020


Terminator.png

Double Terminator GB compatible with B6-C1

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B6-C1.

Figure 1.The overhangs of this part in the Golden Braid Grammar.
Figure 2. The level 0 module : pupd2- double terminator. (illustration from SnapGene)

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction digestion of Terminator (C3 )with : EcoRI & PstI ( C3 ), Expected bands : 2029 + 209 bp , Positive result : C3