Difference between revisions of "Part:BBa K3629004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | XPR2 terminator is a native <i>Yarrowia lipolytica</i> terminator for the alkaline XPR2 exonulcease gene. XPR2 is among the most common terminators used for heterologous expression of proteins in <i>Y. lipolytica.</i> (1) | |
===Design=== | ===Design=== | ||
Revision as of 06:35, 26 October 2020
Yarrowia lipolytica XPR2 terminator
Terminator sequence from the Yarrowia lipolytica alkaline extracellular protease XRP2 gene.
Usage and Biology
XPR2 terminator is a native Yarrowia lipolytica terminator for the alkaline XPR2 exonulcease gene. XPR2 is among the most common terminators used for heterologous expression of proteins in Y. lipolytica. (1)
Design
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 271
Illegal EcoRI site found at 265 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 265
Illegal SpeI site found at 272
Illegal PstI site found at 286
Illegal NotI site found at 7
Illegal NotI site found at 279 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 265 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 272
Illegal EcoRI site found at 265 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 265
Illegal XbaI site found at 16
Illegal SpeI site found at 272
Illegal PstI site found at 286 - 1000COMPATIBLE WITH RFC[1000]