Difference between revisions of "Part:BBa K3629020"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | Anthranilate synthase component 1 is the first enzyme involved in the biosynthesis of tryptophan This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a tryptophan binding domain which allows for | + | Anthranilate synthase component 1 is the first enzyme involved in the biosynthesis of tryptophan This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway (1). The protein has a tryptophan binding domain which allows for downregulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate tryptophan synthesis and produce a tryptophan overproducing strain. |
See [https://parts.igem.org/Part:BBa_K3629024 BBa_K3629024] for a high expression construct using this coding sequence. | See [https://parts.igem.org/Part:BBa_K3629024 BBa_K3629024] for a high expression construct using this coding sequence. |
Revision as of 04:56, 26 October 2020
Yarrowia lipolytica TRP2 Gene
Anthranilate synthase component 1 (TRP2) coding sequence Yarrowia lipolytica .
Usage and Biology
Anthranilate synthase component 1 is the first enzyme involved in the biosynthesis of tryptophan This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway (1). The protein has a tryptophan binding domain which allows for downregulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate tryptophan synthesis and produce a tryptophan overproducing strain.
See BBa_K3629024 for a high expression construct using this coding sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 943
Illegal NgoMIV site found at 1213
Illegal NgoMIV site found at 1294
Illegal NgoMIV site found at 1393 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 283
Illegal BsaI.rc site found at 544
Illegal BsaI.rc site found at 1441
References
1. [online] https://www.genome.jp/kegg-bin/show_pathway?yli00400 (Accessed October 26, 2020)