Difference between revisions of "Part:BBa K3365053:Design"
(→‎Design Notes)
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
  
−
Be careful especially when designing primers. Due to complex secondary structure of double terminator, we keep a small insignificant sequence downstream of the double terminator as linker for convenient when amplifying this part by PCR.
+
Be careful especially when designing primers. Due to complex secondary structure of double terminator, we keep a small insignificant sequence downstream of double terminator as linker for convenient when amplifying this part by PCR.
−
 
+
  
 
===Source===
 
===Source===

Revision as of 03:15, 26 October 2020


RFP with double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 700
    Illegal XhoI site found at 691
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Be careful especially when designing primers. Due to complex secondary structure of double terminator, we keep a small insignificant sequence downstream of double terminator as linker for convenient when amplifying this part by PCR.

Source

We amplify this part on plasmid pE1a-RFP kindly offered by team Tongji_China.

References