Difference between revisions of "Part:BBa K165061"
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<partinfo>BBa_K165061 short</partinfo> | <partinfo>BBa_K165061 short</partinfo> | ||
− | + | Useful for chromosomal integration of a part into yeast strains. This will insert the vector at the specific locus of the TRP13 gene. To be used in conjunction with tryptophan drop-out media for positive selection of transformed cells. Transformation using this vector requires linearization of the plasmid by cutting with PstI. | |
− | + | To incorporate a single Biobrick part into this vector for subsequent transformation into yeast, one should cut both vector and part with EcoRI and SpeI. | |
− | + | Alternatively, one can do the final ligation step between two Biobrick parts into this vector by cutting with the following: | |
+ | |||
+ | Vector: EcoRI, Not I | ||
+ | Prefix: EcoRI, SpeI | ||
+ | Suffix: XbaI, NotI | ||
+ | |||
+ | |||
+ | Yeast transformation protocol: | ||
+ | R Daniel Gietz & Robert H Schiestl. High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method . Nature protocols (2007) | ||
− | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 00:39, 30 October 2008
Status: 500 Content-type: text/html
Software error:
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Useful for chromosomal integration of a part into yeast strains. This will insert the vector at the specific locus of the TRP13 gene. To be used in conjunction with tryptophan drop-out media for positive selection of transformed cells. Transformation using this vector requires linearization of the plasmid by cutting with PstI.
To incorporate a single Biobrick part into this vector for subsequent transformation into yeast, one should cut both vector and part with EcoRI and SpeI.
Alternatively, one can do the final ligation step between two Biobrick parts into this vector by cutting with the following:
Vector: EcoRI, Not I Prefix: EcoRI, SpeI Suffix: XbaI, NotI
Yeast transformation protocol:
R Daniel Gietz & Robert H Schiestl. High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method . Nature protocols (2007)
Sequence and Features
Status: 500
Content-type: text/html
Software error:
syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 230, near ""<BR>$group_name, $year, $team_name, $team_digits, $range_name, $range_prefix")" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.