Difference between revisions of "Part:BBa K165061"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K165061 short</partinfo>
 
<partinfo>BBa_K165061 short</partinfo>
  
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This is a vector specifically designed for integration into a yeast genome at the LEU2 gene.
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This is a vector specifically designed for integration into a yeast genome at the TRP1 gene.
  
 
The final construction step of a Biobrick device can be made into this vector but cutting with the following scheme:
 
The final construction step of a Biobrick device can be made into this vector but cutting with the following scheme:
  
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The system works in a yeast strain that has a mutation in the LEU2 gene, meaning that it is incapable of producing leucine on its own.  Untransformed yeast cells with this mutation must be grown in media that contains leucine in order to be viable.  However, after a transformation protocol in which competent yeast cells are treated with the pRS304* plasmid
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The system works in a yeast strain that has a mutation in the TRP1 gene, meaning that it is incapable of producing leucine on its own.  Untransformed yeast cells with this mutation must be grown in media that contains leucine in order to be viable.  However, after a transformation protocol in which competent yeast cells are treated with the pRS304* plasmid
  
 
Yeast integration  
 
Yeast integration  

Revision as of 19:48, 29 October 2008

pRS304* yeast shuttle vector, TRP1 selection

This is a vector specifically designed for integration into a yeast genome at the TRP1 gene.

The final construction step of a Biobrick device can be made into this vector but cutting with the following scheme:

The system works in a yeast strain that has a mutation in the TRP1 gene, meaning that it is incapable of producing leucine on its own. Untransformed yeast cells with this mutation must be grown in media that contains leucine in order to be viable. However, after a transformation protocol in which competent yeast cells are treated with the pRS304* plasmid

Yeast integration

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1942
    Illegal XbaI site found at 550
    Illegal XbaI site found at 1912
    Illegal SpeI site found at 1918
    Illegal PstI site found at 251
    Illegal PstI site found at 1936
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1942
    Illegal SpeI site found at 1918
    Illegal PstI site found at 251
    Illegal PstI site found at 1936
    Illegal NotI site found at 1904
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1942
    Illegal BamHI site found at 1924
    Illegal XhoI site found at 1975
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1942
    Illegal XbaI site found at 550
    Illegal XbaI site found at 1912
    Illegal SpeI site found at 1918
    Illegal PstI site found at 251
    Illegal PstI site found at 1936
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1942
    Illegal XbaI site found at 550
    Illegal XbaI site found at 1912
    Illegal SpeI site found at 1918
    Illegal PstI site found at 251
    Illegal PstI site found at 1936
    Illegal NgoMIV site found at 1563
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3348
    Illegal SapI site found at 2265