Difference between revisions of "Part:BBa K3425140:Design"
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The RBS used (BBa_B0034) is an ''E. coli'' part but it is also known to work in ''B. subtilis''. | The RBS used (BBa_B0034) is an ''E. coli'' part but it is also known to work in ''B. subtilis''. | ||
| − | When designing composite parts for this chassis, using a double terminator is recommended. Thus, | + | When designing composite parts for this chassis, using a double terminator is recommended. Thus, BBa_B0015 was used. |
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===Source=== | ===Source=== | ||
The BBa_E0040 sequence was obtained from the registry and BBa_K3425130 from the chromosome of ''B. subtilis'' strain w168. | The BBa_E0040 sequence was obtained from the registry and BBa_K3425130 from the chromosome of ''B. subtilis'' strain w168. | ||
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Latest revision as of 23:32, 25 October 2020
PydfJ115_GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 749
Design Notes
The RBS used (BBa_B0034) is an E. coli part but it is also known to work in B. subtilis.
When designing composite parts for this chassis, using a double terminator is recommended. Thus, BBa_B0015 was used.
Source
The BBa_E0040 sequence was obtained from the registry and BBa_K3425130 from the chromosome of B. subtilis strain w168.