Difference between revisions of "Part:BBa K077557"

Latest revision as of 19:28, 29 October 2008

Part 1 of the Conways game of life system

This part constitutively produces LuxR, which forms a complex with HSL from neighboring cells. These LuxR.HSL complexes bind on the cI+LuxR promoter to produce LuxI, responsible for the HSL production to send signals to its own neighbors. Also GFP will be produced to visualize the ON state.

Input: 3OC6HSL

Output: LuxR, LuxI (producing 3OC6HSL) and GFP

Flow cytometry results of K077557. A total of 20.000 events were measured. Y-axis indicates number of events. X-axis indicates fluorescence on a logarithmic scale. Z-axis indicates time after induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1766
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2442

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K077557 short</partinfo>
-Part 1 of the Conway's Game of Life system.
 This part constitutively produces LuxR, which forms a complex with HSL from neighboring cells. These LuxR.HSL complexes bind on the cI+LuxR promoter to produce LuxI, responsible for the HSL production to send signals to its own neighbors. Also GFP will be produced to visualize the ON state.
 Input: 3OC6HSL
 Output: LuxR, LuxI (producing 3OC6HSL) and GFP
 [[Image:K077557flow.jpg]]
+Flow cytometry results of K077557. A total of 20.000 events were measured. Y-axis indicates number of events. X-axis indicates fluorescence on a logarithmic scale. Z-axis indicates time after induction.
 <!-- Add more about the biology of this part here