Difference between revisions of "Part:BBa K3589202"

Revision as of 17:14, 25 October 2020

L1 � Wildtype marLac + HA (Phytobrick)

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), a 3xHA-tag (BBa_K3002017) and the coding sequence of the wildtype laccase from an uncultured marine bacteria (BBa_K3589109) plus the MoClo connectors for positions B2-B3 ( BBa_K3002303), B4-B5 ( BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1312
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 249
Illegal PstI site found at 1312
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1312
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1312
Illegal NgoMIV site found at 1172
Illegal NgoMIV site found at 1891
Illegal NgoMIV site found at 2342
Illegal NgoMIV site found at 2895
1000
COMPATIBLE WITH RFC[1000]

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-This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), a 3xHA-tag (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>) and the coding sequence of the mutant laccase from an uncultured marine bacteria (<a href="https://parts.igem.org/Part:BBa_K3589109">BBa_K3589109</a>) plus the MoClo connectors for positions B2-B3 ( <a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 ( <a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>).
+This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), a 3xHA-tag (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>) and the coding sequence of the wildtype laccase from an uncultured marine bacteria (<a href="https://parts.igem.org/Part:BBa_K3589109">BBa_K3589109</a>) plus the MoClo connectors for positions B2-B3 ( <a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 ( <a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>).
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