Difference between revisions of "Part:BBa K3467007:Experience"
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From the reference, CarH tetramer can bind with CarO operon and its induction fold can up to be 350. We inserted it in our yeast system with a TEF promoter on its upstream. We've already transformed it into yeast system and we'll do further research to test whether our CarH composite part can work well in the yeast system. | From the reference, CarH tetramer can bind with CarO operon and its induction fold can up to be 350. We inserted it in our yeast system with a TEF promoter on its upstream. We've already transformed it into yeast system and we'll do further research to test whether our CarH composite part can work well in the yeast system. | ||
| + | [[File:T--CAU_China--CarH-VP16_Protein.jpg|500px|thumb|center|'''Fig. 1''' CarH-VP16]] | ||
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| + | In our project, we’ve already transferred our CarH composite part into yeast. We’ll do further research to test whether it will work well in yeast. | ||
1.Chatelle, C. et al., A Green-Light-Responsive System for the Control of Transgene Expression in Mammalian and Plant Cells. ACS SYNTH BIOL 7 1349 (2018). | 1.Chatelle, C. et al., A Green-Light-Responsive System for the Control of Transgene Expression in Mammalian and Plant Cells. ACS SYNTH BIOL 7 1349 (2018). | ||
Latest revision as of 03:02, 27 October 2020
From the reference, CarH tetramer can bind with CarO operon and its induction fold can up to be 350. We inserted it in our yeast system with a TEF promoter on its upstream. We've already transformed it into yeast system and we'll do further research to test whether our CarH composite part can work well in the yeast system.
In our project, we’ve already transferred our CarH composite part into yeast. We’ll do further research to test whether it will work well in yeast.
1.Chatelle, C. et al., A Green-Light-Responsive System for the Control of Transgene Expression in Mammalian and Plant Cells. ACS SYNTH BIOL 7 1349 (2018).