Difference between revisions of "Part:BBa K3589150"
Line 3: Line 3:
 
<partinfo>BBa_K3589150 short</partinfo>
 
<partinfo>BBa_K3589150 short</partinfo>
  
−
This basic part contains the sequence of the SP20-HA-RGS-8His-tag (B5). Combined with a secretion signal of the Kaiser Collection from TU Kaiserslauterns 2019 iGEM Team like BBa_K3002007 (cCA (B2)), BBa_BBa_K3002008 (GLE (B2)) or BBa_3002009 (ARS (B2)), an appropriate promoter (BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))) and a terminator (e.g. BBa_BBa_K3002006(RPL23 terminator)) your target protein should be secreted efficiently and you should be able to detect it via HA-antibody and purify it via a nickel-NTA column.
+
<html>
−
The SP20 tag should improve the secretion of proteins by increasing the glycosylation. It is a repetition of Prolin and Serin, whereby Prolin is converted into hydroxyproline and then glycolyzed.
+
<p>
−
 
+
This basic part contains the sequence of the SP20-HA-RGS-8His-tag (B5). Combined with a secretion signal of the Kaiser Collection from TU Kaiserslauterns 2019 iGEM Team like <a href="https://parts.igem.org/Part:BBa_K3002007">BBa_K3002007</a> (cCA (B2)), <a href="https://parts.igem.org/Part:BBa_K3002008">BBa_K3002008</a> (GLE (B2)) or <a href="https://parts.igem.org/Part:BBa_K3002009">BBa_3002009</a> (ARS (B2)), an appropriate promoter (<a href="https://parts.igem.org/Part:BBa_K3002001">BBa_K3002001 </a>(PSAD promoter (A1-B1)) or <a href="https://parts.igem.org/Part:BBa_K3002031">BBa_K3002031</a> (PAR promoter (A1-B1))) and a terminator (e.g. <a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a> (RPL23 terminator)) your target protein should be secreted efficiently and you should be able to detect it via HA-antibody and purify it via a nickel-NTA column.<br>
 +
The SP20 tag should improve the secretion of proteins by increasing the glycosylation. It is a repetition of Prolin and Serin, whereby Prolin is converted into hydroxyproline and then glycolyzed (<a href="https://doi.org/10.1111/pbi.12710">Ramos-Martinez <I>et. al</I>, 2017</a>).
 +
</p>
 +
</html>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 16:47, 25 October 2020


SP20-HA-RGS-8xHis tag for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the sequence of the SP20-HA-RGS-8His-tag (B5). Combined with a secretion signal of the Kaiser Collection from TU Kaiserslauterns 2019 iGEM Team like BBa_K3002007 (cCA (B2)), BBa_K3002008 (GLE (B2)) or BBa_3002009 (ARS (B2)), an appropriate promoter (BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))) and a terminator (e.g. BBa_K3002006 (RPL23 terminator)) your target protein should be secreted efficiently and you should be able to detect it via HA-antibody and purify it via a nickel-NTA column.
The SP20 tag should improve the secretion of proteins by increasing the glycosylation. It is a repetition of Prolin and Serin, whereby Prolin is converted into hydroxyproline and then glycolyzed (Ramos-Martinez et. al, 2017).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]