Difference between revisions of "Part:BBa K3562000"
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Different AHL degrading enzymes have different substrate preferences and degradation activities.(Fig.2) | Different AHL degrading enzymes have different substrate preferences and degradation activities.(Fig.2) | ||
[[Image:AHL-lactonase.png|frame|'''Figure 1:'''Mechanism of AHL-lactonase]] | [[Image:AHL-lactonase.png|frame|'''Figure 1:'''Mechanism of AHL-lactonase]] | ||
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Revision as of 15:56, 25 October 2020
AiiC (with His-tag)
AHL-lactonase with AHLs (Quorum-sensing factor Acyl Homoserine Lactone) degradation activity.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 2071
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2071
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 2071
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 2071
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
AHL-lactonase is a kind of lactonase opened the homoserine lactone ring of AHL in the presence of water to produce N-acly homoserine.(Fig.1) The biological activity of degradation products will be greatly reduced.
Quorum sensing factor is closely related to bacterial virulence. Enzymes that degrade AHL can control the concentration of AHL to silence the expression of related genes. In our project, we use these enzymes to control the virulence of Pseudomonas aeruginosa referred to existing experimental therapies.
Different AHL degrading enzymes have different substrate preferences and degradation activities.(Fig.2)