Difference between revisions of "Part:BBa K3425056"
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<partinfo>BBa_K3425056 short</partinfo> | <partinfo>BBa_K3425056 short</partinfo> | ||
− | + | BBa K3425056 is a T7 polymerase regulated expression construct of Juniper GFP constructed from the weaker promoter, BBa_K3425035, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015. | |
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Latest revision as of 00:11, 28 October 2020
T7 polymerase regulated expression construct of Juniper GFP
BBa K3425056 is a T7 polymerase regulated expression construct of Juniper GFP constructed from the weaker promoter, BBa_K3425035, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 461
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 461
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 461
Illegal BamHI site found at 670 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 461
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 461
- 1000COMPATIBLE WITH RFC[1000]