Difference between revisions of "Part:BBa K3425056"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K3425056 short</partinfo>
 
<partinfo>BBa_K3425056 short</partinfo>
  
−
TBD
+
BBa K3425056 is a T7 polymerase regulated expression construct of Juniper GFP constructed from the weaker promoter, BBa_K3425035, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:11, 28 October 2020


T7 polymerase regulated expression construct of Juniper GFP

BBa K3425056 is a T7 polymerase regulated expression construct of Juniper GFP constructed from the weaker promoter, BBa_K3425035, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 461
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 461
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 461
    Illegal BamHI site found at 670
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 461
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 461
  • 1000
    COMPATIBLE WITH RFC[1000]