Difference between revisions of "Part:BBa K3396015"
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===Usage and Biology===
 
===Usage and Biology===
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This part was designed by us to add antibody conveniently. Two restriction sites of AlwI were added to the 5’ and 3’ end of GFPnano, through which, the binding protein of any target gene can be inserted using Golden-Gate Assembly, with no worry about the multiple illegal sites contained in it.  
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This part was designed by us to add replace modules in the PREDATOR Pro system conveniently. Two AlwI sites of were added to the 5' and 3’ end of XTEN Linker, through which, other coding sequences can be inserted using Golden-Gate Assembly.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 00:26, 27 October 2020


Repleceable-3

This is the antibody of target protein with specific restriction sites that could be recognized by AlwI.

Usage and Biology

This part was designed by us to add replace modules in the PREDATOR Pro system conveniently. Two AlwI sites of were added to the 5' and 3’ end of XTEN Linker, through which, other coding sequences can be inserted using Golden-Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]