Difference between revisions of "Part:BBa K3380810"

 
 
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<partinfo>BBa_K3380810 short</partinfo>
 
<partinfo>BBa_K3380810 short</partinfo>
  
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Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by the cnrH transcription factor. Upon addition of Cobalt or Nickel, the promoter allows the polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.
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===Usage and Biology===
 
===Usage and Biology===
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Can be used in a cell free transcription only biosensor
  
 
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Latest revision as of 23:01, 27 October 2020


TXO cnrH regulated construct under BBa_K3380110 promoter expressing iSpinach

Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by the cnrH transcription factor. Upon addition of Cobalt or Nickel, the promoter allows the polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.


Usage and Biology

Can be used in a cell free transcription only biosensor

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]