Difference between revisions of "Part:BBa K3657022"
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<partinfo>BBa_K3657022 short</partinfo> | <partinfo>BBa_K3657022 short</partinfo> | ||
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<p>!!!This part works in combination with the part <a href= "https://parts.igem.org/Part:BBa_K3657023">BBa_K3657023</a>.!!!</p> | <p>!!!This part works in combination with the part <a href= "https://parts.igem.org/Part:BBa_K3657023">BBa_K3657023</a>.!!!</p> | ||
<p>It was proved by Knucker et al. that the transcript of this sequence creates a triple helix with the DNA part (BBa_K3657023) in vitro.</p> | <p>It was proved by Knucker et al. that the transcript of this sequence creates a triple helix with the DNA part (BBa_K3657023) in vitro.</p> | ||
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<p>More information available here: LINK</p> | <p>More information available here: LINK</p> | ||
<h4>Usage and Biology</h4> | <h4>Usage and Biology</h4> | ||
| − | + | <p> Triple helices are one of the alternative structures formed by nucleic acids. In addition to the Watson-Crick interactions between base pairs, Hoogsteen interactions are crucial for the formation of the triple helix. </p> | |
<h4>Part Design</h4> | <h4>Part Design</h4> | ||
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<li>Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.</li> | <li>Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.</li> | ||
</ol> | </ol> | ||
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</p> | </p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3657022 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3657022 SequenceAndFeatures</partinfo> | ||
Revision as of 14:28, 25 October 2020
RNA Part of an RNA·DNA-DNA triple helix
!!!This part works in combination with the part BBa_K3657023.!!!
It was proved by Knucker et al. that the transcript of this sequence creates a triple helix with the DNA part (BBa_K3657023) in vitro.
More information available here: LINK
Usage and Biology
Triple helices are one of the alternative structures formed by nucleic acids. In addition to the Watson-Crick interactions between base pairs, Hoogsteen interactions are crucial for the formation of the triple helix.
Part Design
You can generate analogical parts yourself as following:
- Generate a random DNA sequence containing adenine, cytosine and guanine. You sequence should be longer than 19 base pairs and preferably should not contain thymine.
- Use the following script to generate RNA: LINK. Corresponding bases in the script are the pairs that create strongest bonds according to Knucker et al.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]